Technical data

59
SureSelect
XT
Target Enrichment System for Illumina Paired-End
Sequencing Library Protocol
Agilent Technologies
4
Hybridization and Capture
Step 1. Hybridize DNA samples to the Capture Library 60
Step 2. Prepare streptavidin-coated magnetic beads 66
Step 3. Capture the hybridized DNA using streptavidin-coated beads 67
This chapter describes the steps to hybridize the prepared gDNA libraries
with a target-specific Capture Library. After hybridization, the targeted
molecules are captured on streptavidin beads.
Each DNA library sample must be hybridized and captured individually
prior to addition of the indexing tag by PCR.
CAUTION
The ratio of Capture Library to gDNA library is critical for successful capture.