Technical data

Sample Preparation (200 ng DNA Samples) 3
Step 11. Assess quality and quantity
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 57
Step 11. Assess quality and quantity
Sample analysis can be done with either the 2100 Bioanalyzer instrument
or the 2200 TapeStation instrument.
Option 1: Analysis using the 2100 Bioanalyzer and DNA 1000 Assay
See the DNA 1000 Kit Guide at www.genomics.agilent.com for more
information on doing this step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.02 or higher), turn on the
2100 Bioanalyzer and check communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the DNA 1000 assay from the
drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Verify that the electropherogram shows a distribution with a DNA
fragment size peak of approximately 225 to 275 bp. Determine the
concentration of the library DNA by integrating under the peak. A
sample electropherogram is shown in Figure 7.
Figure 7 Analysis of amplified library DNA using a DNA 1000 Bioanalyzer assay.