Technical data

Sample Preparation (200 ng DNA Samples) 3
Step 9. Amplify the adaptor-ligated library
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 55
3 Run the program in Table 29 in a thermal cycler.
.
Table 29 Pre-Capture PCR Thermal Cycler Program
Segment Number of
Cycles
Temperature Time
1 1 98°C 2 minutes
2 10 98°C 30 seconds
65°C 30 seconds
72°C 1 minute
3 1 72°C 10 minutes
4 1 4°C Hold