Technical data

Sample Preparation (200 ng DNA Samples) 3

Step 9. Amplify the adaptor-ligated library

SureSelect

Target Enrichment System for Illumina Multiplexed Sequencing 53

Step 9. Amplify the adaptor-ligated library

This step uses the components listed in Table 27. Thaw the reagents listed

below and keep on ice.

Table 27 Reagents for pre-capture PCR amplification

Component Storage Location

SureSelect Primer (brown cap) SureSelect XT Library Prep Kit ILM, –20°C

SureSelect ILM Indexing Pre-Capture PCR

Reverse Primer (clear cap)

SureSelect Target Enrichment Kit ILM Indexing

Hyb Module Box 2, –20°C

Herculase II Fusion DNA Polymerase (red cap) Herculase II Fusion DNA Polymerase kit, –20°C

5× Herculase II Reaction Buffer (clear cap) Herculase II Fusion DNA Polymerase kit

, –20°C

* Do not use the PCR Reaction Buffer or dNTP mix from any other kit.

100 mM dNTP Mix (green cap) Herculase II Fusion DNA Polymerase kit*, –20°C