Technical data

SureSelect

Target Enrichment System for Illumina Multiplexed Sequencing 5

Content

1 Before You Begin 9

Overview of the Workflow 10

Procedural Notes 12

Safety Notes 13

Required Reagents 14

Optional Reagents 16

Required Equipment 17

Optional Equipment 19

2 Sample Preparation (3 µg DNA Samples) 21

Step 1. Shear the DNA 22

Step 2. Purify the sample using AMPure XP beads 24

Step 3. Assess quality (optional) 26

Step 4. Repair the ends 28

Step 5. Purify the sample using AMPure XP beads 29

Step 6. Adenylate the 3' end of the DNA fragments 30

Step 7. Purify the sample using AMPure XP beads 31

Step 8. Ligate the paired-end adaptor 32

Step 9. Purify the sample using AMPure XP beads 33

Step 10. Amplify the adaptor-ligated library 34

Step 11. Purify the amplified library with AMPure XP beads 37

Step 12. Assess quality and quantity 38