Technical data

Sample Preparation (3 µg DNA Samples) 2
Step 12. Assess quality and quantity
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 39
Option 2: Analysis using the 2200 TapeStation and D1000 ScreenTape
For more information to do this step, see the Agilent 2200 TapeStation
User Manual at www.genomics.agilent.com.
1 Prepare t
he TapeStation samples as instructed in the Agilent 2200
TapeStation User Manual. Use 1 µL of each DNA sample diluted with
3 µL of D1000 sample buffer for the analysis.
2 Load the sample plate or tube strips from step 1, the D1000
ScreenTape, and loading tips into the 2200 TapeStation as instructed in
the Agilent 2200 TapeStation User Manual. Start the run.
3 Verify that the electropherogram shows a distribution with a DNA
fragment size peak of approximately 225 to 275 bp. Determine the
concentration of the library DNA by integrating under the peak. A
sample electropherogram is shown in Figure 5.
Figure 5 Analysis of amplified library DNA using a D1000 ScreenTape.
CAUTION
Make sure that you thoroughly mix the combined DNA and sample buffer on a vortex
mixer for 5 seconds for accurate quantitation.