Technical data

2 Sample Preparation (3 µg DNA Samples)
Step 10. Amplify the adaptor-ligated library
34 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
Step 10. Amplify the adaptor-ligated library
This step uses the components listed in Table 16. Thaw the reagents listed
below and keep on ice.
This protocol uses half of the adaptor-ligated library for amplification. The
remainder can be saved at –20°C for future use, if needed.
Table 16 Reagents for pre-capture PCR amplification
Component Storage Location
SureSelect Primer SureSelect XT Library Prep Kit ILM, –20°C
SureSelect ILM Indexing Pre-Capture PCR
Reverse Primer
SureSelect Target Enrichment Kit ILM Indexing
Hyb Module Box 2, –20°C
Herculase II Fusion DNA Polymerase Herculase II Fusion DNA Polymerase kit, –20°C
5× Herculase II Reaction Buffer Herculase II Fusion DNA Polymerase kit
*
, –20°C
* Do not use the PCR Reaction Buffer or dNTP mix from any other kit.
100 mM dNTP Mix Herculase II Fusion DNA Polymerase kit*, –20°C