Technical data

SureSelect

Target Enrichment System for Illumina Multiplexed Sequencing 3

In this Guide...

This guide provides an optimized protocol for Illumina

paired-end multiplexed library preparation using the

SureSelect

Library Prep and Capture System.

This guide describes the recommended operational

procedures to capture the genomic regions of interest using

biotinylated RNA oligomer libraries to enrich targeted

regions of the genome from repetitive sequences and

sequences unrelated to the research focus prior to sample

sequencing using the Illumina platform.

1 Before You Begin

This chapter contains information (such as procedural notes,

safety information, required reagents and equipment) that

you should read and understand before you start an

experiment.

2 Sample Preparation (3 µg DNA Samples)

This chapter describes the steps to prepare libraries for

target enrichment from 3-µg gDNA samples.

3 Sample Preparation (200 ng DNA Samples)

This chapter describes the steps to prepare libraries for

target enrichment from 200-ng gDNA samples.

4 Hybridization and Capture

This chapter describes the steps to hybridize and capture

the prepared library DNA.

5 Indexing and Sample Processing for Multiplexed Sequencing

This chapter describes the steps to amplify, purify, and

assess quality and quantity of the sample libraries. Samples

are pooled by mass prior to sequencing.

6 Reference

This chapter contains reference information, including

component kit contents and index sequences.