Technical data

Sample Preparation (3 µg DNA Samples) 2
Step 3. Assess quality (optional)
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 27
Option 2: Analysis using the 2200 TapeStation and D1000 ScreenTape
Use a D1000 ScreenTape and D1000 Reagents for analysis of the 3 g
sheared DNA samples using the 2200 TapeStation. For more information
to do this step, see the Agilent 2200 TapeStation User Manual at
www.genomics.agilent.com.
1 Prepare t
he TapeStation samples as instructed in the Agilent 2200
TapeStation User Manual. Use 1 µL of each DNA sample diluted with
3 µL of D1000 sample buffer for the analysis.
2 Load the sample plate or tube strips from step 1, the D1000
ScreenTape, and loading tips into the 2200 TapeStation as instructed in
the Agilent 2200 TapeStation User Manual. Start the run.
3 Verify that the electropherogram shows a DNA fragment size peak
between 150–200 bp. A sample electropherogram is shown in Figure 3.
Figure 3 Analysis of sheared DNA using a D1000 ScreenTape.
CAUTION
Make sure that you thoroughly mix the combined DNA and sample buffer on a vortex
mixer for 5 seconds for accurate quantitation.