Technical data

2 Sample Preparation (3 µg DNA Samples)
Step 3. Assess quality (optional)
26 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
Step 3. Assess quality (optional)
This step is optional.
Quality assessment can be done with either the 2100 Bioanalyzer
instrument or the 2200 TapeStation instrument.
Option 1: Analysis using the 2100 Bioanalyzer and DNA 1000 Assay
Use a DNA 1000 chip and reagent kit for analysis of the 3 g sheared
DNA samples using the 2100 Bioanalyzer. See the DNA 1000 Kit Guide at
www.genomics.agilent.com for more information on doing this step.
1 Check that the 2100 Bioanalyzer electrodes have been cleaned as
instructed in the reagent kit guide.
2 Open the 2100 Expert Software (version B.02.02 or higher), turn on the
2100 Bioanalyzer and check communication.
3 Prepare the chip, samples and ladder as instructed in the reagent kit
guide, using 1 µL of each sample for the analysis.
4 Load the prepared chip into the 2100 Bioanalyzer and start the run
within five minutes after preparation.
5 Within the instrument context, choose the DNA 1000 assay from the
drop down list.
6 Start the run. Enter sample names and comments in the Data and
Assay context.
7 Check that the electropherogram shows a DNA fragment size peak
between 150–200 bp. A sample electropherogram is shown in Figure 2.
Figure 2 Analysis of sheared DNA using a DNA 1000 Bioanalyzer assay.