Technical data

Sample Preparation (3 µg DNA Samples) 2
Step 2. Purify the sample using AMPure XP beads
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 25
Use fresh 70% ethanol for optimal results.
9 Wait for 1 minute to allow any disturbed beads to settle, then remove
the ethanol.
10 Repeat step 8 to step 9 once.
11 Seal the wells with strip caps, then briefly spin the plate to collect the
residual ethanol. Return the plate to the magnetic stand for 30 seconds.
Remove the residual ethanol with a P20 pipette.
12 Dry the samples by placing the unsealed plate on the thermal cycler, set
to hold samples at 37°C, for 3 to 5 minutes or until the residual
ethanol completely evaporates.
Do not dry the bead pellet to the point that the pellet appears cracked during any of the
bead drying steps in the protocol. Elution efficiency is significantly decreased when the
bead pellet is excessively dried.
13 Add 50 µL nuclease-free water to each sample well.
14 Seal the wells with strip caps, then mix well on a vortex mixer and
briefly spin the plate to collect the liquid.
15 Incubate for 2 minutes at room temperature.
16 Put the plate in the magnetic stand and leave for 2 to 3 minutes, until
the solution is clear.
17 Remove the cleared supernatant (approximately 48 µL) to a fresh PCR
plate well. You can discard the beads at this time.
Stopping Point If you do not continue to the next step, seal the plate and store at –20°C.
NOTE