Technical data

2 Sample Preparation (3 µg DNA Samples)

Step 2. Purify the sample using AMPure XP beads

24 SureSelect

Target Enrichment System for Illumina Multiplexed Sequencing

Step 2. Purify the sample using AMPure XP beads

Instructions in this protocol are for sample processing in 96-well PCR plates. When

processing a small number of samples, you can instead use strip tubes or individual tubes

that are compatible with the thermal cycler and magnetic separation device used in the

protocol.

1 Let t

he AMPure XP beads come to room temperature for at least

30 minutes. Do not freeze the beads at any time.

2 Prepare 400 µL of 70% ethanol per sample, plus excess, for use in

step 8.

The freshly-prepared 70% ethanol may be used for subsequent purification steps run on the

same day. The complete Library Preparation protocol requires 2 mL of fresh 70% ethanol

per sample.

3 Mix the bead suspension well so that the reagent appears homogeneous

and consistent in color.

4 Add 180 µL of homogeneous AMPure XP beads to each sheared DNA

sample (approximately 130 µL) in the PCR plate. Pipette up and down

10 times to mix.

5 Incubate samples for 5 minutes at room temperature.

6 Put the plate into a magnetic separation device. Wait for the solution to

clear (approximately 3 to 5 minutes).

7 Keep the plate in the magnetic stand. Carefully remove and discard the

cleared solution from each well. Do not touch the beads while removing

the solution.

8 Continue to keep the plate in the magnetic stand while you dispense

200 µL of 70% ethanol in each sample well.

NOTE

If some magnetic beads remain suspended in solution after 5 minutes, carefully remove and

discard 100 l of cleared solution from near the bottom of the wells, and continue

incubating the plate in the magnetic stand for an additional 3 minutes. After the remaining

suspension has cleared, remove and discard the remaining cleared solution (approximately

210 l) from the wells.