Technical data

Sample Preparation (3 µg DNA Samples) 2
Step 1. Shear the DNA
SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing 23
6 Secure the microTube in the tube holder and shear the DNA with the
settings in Table 8 or Table 9, depending on the Covaris instrument
SonoLab software version used.
The target DNA fragment size is 150 to 200 bp.
7 Put the Covaris microTube back into the loading and unloading station.
8 While keeping the snap-cap on, insert a pipette tip through the
pre-split septa, then slowly remove the sheared DNA.
9 Transfer each sheared DNA sample (approximately 130 µL) to a
separate well of a 96-well plate or strip tube.
Table 8 Shear settings for Covaris instruments using SonoLab software version 7 or
newer
Setting Value
Duty Factor 10%
Peak Incident Power (PIP) 175
Cycles per Burst 200
Treatment Time 360 seconds
Bath Temperature 4° to 8° C
Table 9 Shear settings for Covaris instruments using SonoLab software prior to
version 7
Setting Value
Duty Cycle 10%
Intensity 5
Cycles per Burst 200
Time 6 cycles of 60 seconds each
Set Mode Frequency sweeping
Temperature 4° to 7° C