Technical data

1 Before You Begin
Required Reagents
14 SureSelect
XT
Target Enrichment System for Illumina Multiplexed Sequencing
Required Reagents
Table 2 Required Reagents for SureSelect
XT
Target Enrichment
Description Vendor and part number
SureSelect, ClearSeq or OneSeq Capture Library
*
* SureSelect, ClearSeq, and OneSeq reagents must be used within one year of receipt.
Select the appropriate library from
Table 3 or Table 4
SureSelect
XT
Reagent Kit, Illumina (ILM) platforms*
HiSeq platform, 16 reactions
HiSeq platform, 96 reactions
HiSeq platform, 480 reactions
MiSeq platform, 16 reaction
MiSeq platform, 96 reactions
MiSeq platform, 480 reactions
Agilent
p/n G9611A
p/n G9611B
p/n G9611C
p/n G9612A
p/n G9612B
p/n G9612C
Agencourt AMPure XP Kit
5 mL
60 mL
450 mL
Beckman Coulter Genomics
p/n A63880
p/n A63881
p/n A63882
Herculase II Fusion DNA Polymerase (includes dNTPs and
5× Buffer)
200 Reactions (processes 100 XT libraries)
400 Reactions
Agilent
p/n 600677
p/n 600679
Dynabeads MyOne Streptavidin T1
2 mL
10 mL
100 mL
Life Technologies
p/n 65601
p/n 65602
p/n 65603
1X Low TE Buffer (10 mM Tris-HCl, pH 8.0, 0.1 mM EDTA) Life Technologies p/n 12090-015, or
equivalent
100% Ethanol, molecular biology grade Sigma-Aldrich p/n E7023
Qubit dsDNA HS Assay Kit or
Qubit BR dsDNA Assay Kit
100 assays
500 assays
Life Technologies p/n Q32851
Life Technologies
p/n Q32850
p/n Q32853
Nuclease-free Water (not DEPC-treated) Ambion Cat #AM9930