Specifications

Checklists 3
Hardware Installation 55
 Manual tune explained
 View Tunes utility demonstrated and its importance explained
 PCI Autotune performed (if applicable)
 NCI Autotune performed (if applicable)
 Optimization of reagent gas and application dependency on gas flow, source
temperature, and emission current discussed
 Importance of retaining tuning and maintenance records discussed
 Importance of backing up data, including tune files
Methods
 Edit entire method demonstrated with OFN_SN.M, explaining the GC and
MS Scan Parameters.
 Method save demonstrated
Data acquisition
 Analytical method developed to run both scan and SIM
 Steps involved in acquiring data and editing an entire method described
 File designated to store the data; data file naming demonstrated
 Acquisition parameters saved as part of a method
 Use Scan/SIM (amu range 50:300/amu ion 272) on OFN for acquisition
 Snapshot demonstrated
Data analysis
 Screen layout explained (TIC, spectral windows, command line, title bar,
etc.)
 Loading data files demonstrated
 TIC, spectra, and extracted ion profiles demonstrated
 Integration performed and explained