Pipette Accuracy Tester PT-Series AD-4212B-PT AD-4212A-PT FX-300i-PT INSTRUCTION MANUAL 1WMPD4001794D
© 2015 A&D Company, Limited. All rights reserved. No part of this publication may be reproduced, transmitted, transcribed, or translated into any language in any form by any means without the written permission of A&D Company, Limited. The contents of this manual and the specifications of the instrument covered by this manual are subject to change for improvement without notice.
CONTENTS 1. INTRODUCTION .............................................................................................................................2 1-1 Unpacking the Tester.......................................................................................................................... 2 1-2 Description of Each Component......................................................................................................... 3 1-3 WinCT-Pipette, Software for Pipette Accuracy Testing..............
1. INTRODUCTION The PT series Pipette Accuracy Tester performs an accuracy test of the volume of a pipette in accordance with ISO 8655. This manual describes how the PT series Pipette Accuracy Tester works and how to get the most out of it in terms of performance. It includes how to assemble the tester, how to measure and how to use the software, WinCT-Pipette, for pipette accuracy testing. Read this manual thoroughly before using the tester and keep it at hand for future reference.
FX-300i-PT 1-2 Description of Each Component Evaporation trap A chamber to prevent the evaporation of the dispensed test liquid (distilled water). (Refer to “1-4 Function of the Evaporation Trap”.) Sample cup (Two types: 5 mL and 30 mL) A measuring cup to contain the test liquid dispensed from a pipette, with a water-absorbent sheet installed, to prevent water droplets from remaining on the edge of the pipette tip. Test liquid cup A container to keep a test liquid such as distilled water.
1-3 WinCT-Pipette, Software for Pipette Accuracy Testing Features The mass value of the distilled water dispensed from a pipette is transmitted from the balance to a PC. Using the temperature of the test liquid and the barometric pressure that are previously entered, the PC calculates the conversion factor (Z factor) from a mass value to a volumetric value and converts the mass value into a volumetric value automatically, using the Z factor.
1-4 Function of the Evaporation Trap In the pipette accuracy test using the gravimetric method, to obtain the mass of the test liquid, distilled water is dispensed from the pipette into the sample cup installed on the balance. The obtained value, however, is generally smaller due to evaporation loss of the test liquid. To prevent evaporation of the test liquid, the evaporation trap is used. The ISO 8655 standard recommends that the humidity of the measurement environment be greater than 50% RH.
2. ASSEMBLING THE TESTER 2-1 AD-4212B-PT / AD-4212A-PT 1. Place the weighing unit on a solid table. For details on the installation site, refer to “3. PRECAUTIONS Display BEFORE USE”. Connection cable Secure the display to the stand using the angle adjustment knobs. Connect the display and the weighing unit using the connection cable. Weighing unit Stand Angle adjustment knob 2. Install the evaporation trap base on the weighing Evaporation trap base unit. 3.
4. Install the lower part of the evaporation trap on the weighing unit, mating the tabs with the holes on the Lower part of the evaporation trap base. Tab Hole 5. Attach the upper part of the evaporation trap to the lower part, mating the tab with the notch. Upper part of the evaporation trap Tab Lower part of the evaporation trap Notch 6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped surface is faced to allow easy operation.
2-2 FX-300i-PT 1. Place the balance on a solid table. For details on the installation site, refer to “3. PRECAUTIONS BEFORE USE”. . 2. Install the fitting boss on the balance, then install Evaporation trap base the evaporation trap base on the fitting boss. Fitting boss 3. Install the sample cup holder (30 mL) on the base. 5 mL sample cup When the 30 mL sample cup is used: Insert the sample cup into the holder. NOTE The actual capacity of the 30 mL Sample cup holder (5 mL) sample cup is 25 mL.
4. Install the lower part of the evaporation trap on the weighing unit, mating the tabs with the holes on the Lower part of the evaporation trap base. Tab Hole 5. Attach the upper part of the evaporation trap to the lower part, mating the tab with the notch. Upper part of the evaporation trap Tab Lower part of the evaporation trap Notch 6. Adjust the direction of the evaporation trap so that the pipette insertion opening on the sloped surface is faced to allow an easy operation.
3. PRECAUTIONS BEFORE USE To get the optimum performance from the balance and acquire accurate weighing data, note the following, especially when using the AD-4212B-PT / AD-4212A-PT with a minimum weighing value of 0.001 mg / 0.1 mg. Install the balance where the measurement environment is appropriate. The best operating temperature is 15°C to 30°C ±0.5°C with greater than 50% RH. Install the balance where it is not exposed to direct sunlight and it is not affected by air from heaters or air conditioners.
4. ANTI-STATIC TREATMENT An anti-static treatment has been applied to the evaporation trap. Cleaning it with alcohol will reduce the effect of the treatment. If the evaporation trap has acquired a static charge, apply an anti-static agent or use the optional AD-1683 DC static eliminator to remove the static electricity. 5. INSTALLING WinCT-Pipette For detailed information on the WinCT-Pipette program, refer to “How to use WinCT-Pipette.pdf ” in the WinCT-Pipette CD-ROM.
6. CONNECTING A PC TO THE BALANCE To make a connection between a PC and the balance, use the RS-232C cable or use both the RS-232C cable and the USB converter. 6-1 Connecting a PC When a PC COM port is used When using the COM port of a PC to make a connection to the balance, use the accessory RS-232C cable.
6-2 Checking COM Port Numbers COM port numbers vary depending on the PC used. Use the Windows Device Manager to check COM port numbers. NOTE When the USB converter is used, check the COM port number with the USB converter connected. 1 Click [Start], [Settings] and [Control Panel]. 2 Double-click [System]. 3 Click [Hardware] and [Device Manager]. 4 Double-click [Port (COM & LPT)]. COM port numbers will be displayed. When the USB converter is used, “ATEN USB to Serial Bridge (COMx)” appears.
7. CALIBRATING THE BALANCE For details on calibration, read the balance instruction manual in addition to this manual. Before calibration, refer to “3. PRECAUTIONS BEFORE USE”. Especially when calibrating the AD-4212B-PT / AD-4212A-PT with a minimum weighing value of 0.001 mg / 0.1 mg, make sure that the installation environment is appropriate and the balance is warmed up for the appropriate duration. 7-1 AD-4212B-PT / AD-4212A-PT 1.
7-2 FX-300i-PT 1. Remove the 30 mL sample cup and leave Upper part of the evaporation trap only the sample cup holder (30 mL) inside the evaporation trap as shown to the right. Lower part of the evaporation trap 2. Press and hold the CAL key until Calout is displayed, then release the CAL key. 3. When Cal 0 Sample cup holder (30 mL) is displayed, press the PRINT key to enter the zero point. Tab (4 in all) 4. After the zero point is entered, the calibration weight value 100 is displayed.
8. PREPARATION BEFORE USE The measurement environment recommended by ISO 8655 is as follows. Temperature: Constant between 15°C and 30°C, changes in temperature ±0.5°C. Humidity: Greater than 50% RH 8-1 Filling the Evaporation Trap with Water Remove the evaporation trap from the balance. Separate the upper and lower part of the evaporation trap.
8-4 Using the Thermometer Each part name LCD Battery cover [ON/OFF] button [AUTO OFF] button [MAX/MINI] button Sheath holder Sheath Material: Main body, battery cover: Acrylonitrile-butadiene- styrene (ABS) Button: Rubber Display: Polycarbonate (PC) Sheath: SUS304 Sheath holder: Polycarbonate (PC) Sheath holder fitting: Iron (Nickel plating) Display Auto power off function Maximum value display Minimum value display Measurement temperature display (Maximum value /Minimum value display) Low battery
Replacing The Battery The battery is not installed when you purchase the thermometer. Properly install the battery in the thermometer by the following procedure. Also, when the “ ” indicator appears at the bottom right in the display, replace the old battery with a new one by the following procedure. * Purchase the battery at your local convenience store, supermarket, hardware store or electrical store. * When the main body is damp, wipe the moisture fully and replace the battery.
Measuring the temperature 1. Press the [ON/OFF] button to turn the power on. 2. Remove the sheath holder when it is attached to the sheath. 3. Measure the temperature by one of the following methods depending on the material to be measured. When measuring the liquid Immerse the sensor into the position where you would like to measure the temperature. When there is steam arising from the liquid, handle the thermometer so that the display is not exposed to the steam.
When measuring the gas Place the sensor in the location where you would like to measure the temperature and wait until the display becomes stable. When measuring a high temperature atmosphere such as steam, use a shield to protect the display from high temperature. Gas Shield CAUTION The thermometer detects a temperature at the tip of the sheath. To protect the thermometer from damage, do not expose parts other than the sheath to high or low temperatures of the substances to be measured.
The thermometer automatically displays the current measurement temperature if no operation is made for 30 seconds while on the maximum or minimum temperature display To check the maximum and minimum temperatures for a new measurement, reset the memory before starting the measurement as described in “Resetting the maximum and minimum temperatures stored in memory” below. CAUTION The maximum and minimum temperatures stored in memory are reset automatically when the battery is removed from the thermometer.
How to use the sheath holder Use the sheath holder to protect the sheath when you carry the thermometer. Be sure the sheath holder is removed from the sheath when performing measurements. To prevent the sheath holder from deformation or damage, before using the sheath holder, allow the sheath to return to room temperature after high or low temperature measurements. Daily care Accurate measurements cannot be made if the temperature detection part is dirty.
9. MEASUREMENT 9-1 Measuring Procedure Using the predetermined pipette volume, perform the measurement the specified times. ISO 8655 recommends ten measurements for each for the volumes described below. At the nominal volume of a fixed volume pipette At the following three points of an adjustable volume pipette 1. 10% of the maximum volume or the lower limit of the adjustable volume range, whichever is greater. 2. 50% of the maximum volume 3.
To reduce errors caused by water temperature Use the correct Z factor. To obtain the correct Z factor, measure the water temperature using the accessory thermometer and enter the value in WinCT-Pipette. For a stable water temperature, allow the temperature of the test liquid to acclimatize to the measurement environment for an appropriate duration of time (ISO 8655 recommends two hours or more) before measurement. Vibration Vibration yields unstable measurement values.
10. AD-4212B-PT / AD-4212A-PT The AD-4212B-PT / AD-4212A-PT is designed exclusively as a pipette accuracy tester. Although the performance as a balance is the same as the AD-4212B / AD-4212A production weighing unit, some of the specifications are different. 10-1 Factory Settings When shipped, the factory settings of the AD-4212B-PT / AD-4212A-PT as a pipette accuracy tester are as follows. Unit Weighing unit: mg Minimum weighing value for the AD-4212B-PT: 0.
10-2 Display Lock Function To use this function, set “Display lock function (ploc)” to “ON (1)”. Why use this function This function is used to compensate time-elapsed errors caused by evaporation. Even when the evaporation trap is used, an evaporation of 0.05 mg per minute (0.05 L per minute) will occur. This evaporation influences the measurement, particularly when the volume of the test liquid is small. For such a situation, the display lock function is useful.
11. TROUBLESHOOTING Displayed values are unstable. The AD-4212B-PT / AD-4212A-PT has a minimum weighing value of 0.001 mg / 0.1 mg and is influenced by the measurement environment. Measures to take Use the accessory breeze break. Referring to “3. PRECAUTIONS BEFORE USE”, check the measurement environment for sources of drafts, vibration and static electricity. The AD-4212B-PT with a minimum weighing value of 0.001 mg is prone to low-frequency vibration such as shaking buildings.
12. SPECIFICATIONS Weighing capacity *1 AD-4212B-PT AD-4212A-PT FX-300i-PT 110 g / 31 g / 5.1 g *2 110 g 320 g Minimum weighing 0.1 mg / 0.01 mg / 0.001 mg 0.1 mg 1 mg value Linearity ±0.2 mg / ±0.05 mg / ±0.05 mg ±0.3 mg ±2 mg Repeatability 0.1 mg / 0.05 mg / 0.015 mg 0.15 mg 1 mg (Standard deviation) Dimensions Weighing unit: 80 (W) x 230 (D) x 200 (H) mm 193 (W) x 262.
12-1 Options Name and components 5-mL sample cup (with a water-absorbent sheet installed) 5-mL sample cup 5 pcs. Lid for 5-mL sample cup 5 pcs Water-absorbent sheet for 5-mL sample cup 5 pcs (The sheet is installed in the cup before shipping.) 30-mL sample cup (with a water-absorbent sheet installed) 30-mL sample cup 5 pcs. Lid for 30-mL sample cup 5 pcs Water-absorbent sheet for 30-mL sample cup 5 pcs (The sheet is installed in the cup before shipping.
13. APPENDIX The table below lists the relation between the ISO 8655 requirements and the PT series pipette accuracy testers. Pipette nominal volume *5 (L) 1 2 5 10 20 50 100 200 500 1000 2000 5000 10000 ISO 8655 requirements Maximum permissible error Balance minimum Accuracy Repeatability weighing value ±% ±L % L mg 5.0 0.05 5 0.05 4.0 0.08 2 0.04 0.001 2.5 0.125 1.5 0.075 1.2 0.12 0.8 0.08 1.0 0.2 0.5 0.1 0.01 1.0 0.5 0.4 0.2 0.8 0.8 0.3 0.3 0.8 1.6 0.3 0.6 0.8 4.0 0.3 1.5 0.8 8.0 0.3 3.0 0.1 0.
MEMO 31
MEMO 32
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3-23-14 Higashi-Ikebukuro, Toshima-ku, Tokyo 170-0013, JAPAN Telephone: [81] (3) 5391-6132 Fax: [81] (3) 5391-6148 A&D ENGINEERING, INC. 1756 Automation Parkway, San Jose, California 95131, U.S.A.
